cheminuminescent protein ladder for western blot

Our featured application notes below provide further details. Utilizing this substrate-free assay, researchers can achieve equivalent sensitivity to traditional chemiluminescence assays while consolidating western blot, ELISA, and other applications onto a single reader. Detect Revert 520 on the Odyssey M Imager. The conjugate has been specifically tested and qualified for Western blot applications. Revert 520 Total Protein Stain is used to stain all sample protein in Western blot lanes so that the total amount of protein in each lane can be used as a basis for accurate and reliable normalization. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. Each type of detection has advantages and disadvantages that need to be considered when selecting a method.Īnother method that we have introduced the ScanLater™ Western Blot Detection System, enables first-of-its-kind western blot detection in a multi-mode microplate reader platform. Revert 520 Total Protein Stain for Western Blot Normalization. For example chemiluminescence can be detected using x-ray film or digital imaging equipment while a fluorescent secondary antibody requires a fluorescence imager.

These methods are described below and require different equipment for detection. There are several western blot methods to consider depending upon the secondary antibody used, detection of the target protein may be colorimetric, chemiluminescent, or fluorescent.

Cheminuminescent protein ladder for western blot